ASTM E2315 Antimicrobial Test / Antimicrobial Testing Services
Standard Guide for Assessment of Antimicrobial Activity Using a Time-Kill (Kill-Time, Time Kill, Kill Time) Study Procedure
ASTM E2315 Time-Kill Test
This test method measures changes of a population of aerobic microorganisms within a specified time period when tested against antimicrobial test materials. There are multiple options to consider when conducting an ASTM E2315 time-kill testing study. These are important to define when conducting this test in order to ensure that certain variables can be distinguished and standardized. These can include:
- Organism selection and growth (see below for more detail)
- Inoculum preparation (see below for more detail)
- Sampling times
The ASTM E2315 Time-Kill Testing procedure allows a comparison analysis of tests done under multiple different and varying environments, circumstances, etc.
This test's main purpose and importance is held in that it can be used to evaluate the decrease of a microbial population of test organisms after being exposed to certain test products and materials.
ASTM E2315 Time-Kill Testing Variants
- transfer to growth media
- transfer to agar plates
- transfer to slants
Inoculum Suspension Preparation and Determination of the Microbial Population
- Broth Suspension
- prepared directly from broth
- prepared from dilute broth
- Agar Plate / Slant
- washing of the microbial growth from agar surface
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