Testing Disinfectants against Salmonella choleraesuis

Testing Disinfectants against Salmonella choleraesuis

Analytical technique: Use-Dilution Method
Microbiological / Use-Dilution Method
Disinfectants

The AOAC Use-Dilution methods, 955.14 (Salmonella enterica), 955.15 (Staphylococcus aureus), and 964.02 (Pseudomonas aeruginosa), are used to measure the efficacy of disinfectants on hard surfaces. AOAC Use Dilution Disinfectant test method is a carrier based test method. Carriers which are stainless steel small cylinders are inoculated with high counts of test organism and the carriers are then dried. These test organism contaminated carriers are then immersed in 10 ml of liquid disinfectant for exposure time advised by the sponsor of the study. After exposure of the disinfectant to the test organism, the carriers are then transferred to a liquid growth media with neutralizer to neutralized the test substance inhibitory action and detect the surviving test organisms.

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AOAC 955.14
AOAC 955.15
AOAC 955.16
AOAC 955.17
AOAC 960.09
AOAC 961.02
AOAC 964.02
AOAC 965.12
AOAC 965.13
AOAC 966.04
AOAC 972.04
AOAC 991.47

AOAC 955.14 Disinfectant Testing