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AATCC 100 Test Method


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Assessment Of Antimicrobial Finishes On Textile Materials

Antimicrobial agents are added to textiles to provide protection against microorganisms. Antimicrobial incorporated textiles prevent infections by harmful microorganism. Assessment of antibacterial finishes on textile materials is determined by the degree of antibacterial activity intended in the use of such materials.
Antimicrobial agents can be incorporated into the threads of the fabric or they can be applied to the surface of the textiles. AATCC 100 test method is carried out to check the antimicrobial efficacy of the fabric whether the textile is washed or No wash condition.

Accugen laboratories test the fabrics prior washing as well as after washing of the fabric to evaluate the persistence of the antimicrobial efficacy of the textile. AATCC 100 test method is carried out for evaluation of antimicrobial efficacy of an antimicrobial fabric.

AATCC 100 antimicrobial test method is a quantitative method in which assessment of antibacterial finishes is determined on the textile materials . Following AATCC 100 test method, Accugen labs, tests the degree of antibacterial activity intended in the use of these materials .


AATCC 100 antimicrobial standard test method is used to quantitatively test the antimicrobial activity of the textiles/ fabrics over the contact period of 24 hours . Other Contact times can be selected .



AATCC 100 test is performed with treated and non-treated (Control) swatches of the test material.


According to AATCC 100 antimicrobial test procedure, Microbial culture is prepared in growth enrichment broth for 24 hours of incubation to obtain high concentration of the test organisms. 1.0 mL of test organism suspension at high concentration is inoculated to antimicrobial treated test sample and untreated control fabric sample.

Organism inoculated fabrics are then incubated for 24 hours under favorable conditions of nutrients and temperature. Bacteria will multiply and will increase in number if fabric is not antimicrobial. Untreated control used in the test will assure the increase in microbial growth. At 0 and 24 hours or other selected time point, organisms are extracted in a neutralizer media which is diluted and plated.

The log reduction and or percent reduction is determined by using Organism survival data from the treated and non-treated materials at that contact time.


Organisms according to AATCC 100 Test:

Staphylococcus aureus and Klebsiella pneumoniae. Additional test organism like E.coli, MRSA, Pseudomonas aeruginosa, Vancomycin resistant enterococci (VRE), Salmonella etc can be tested.


AATCC 100 Test Sample Requirements:
Samples Size: 48 x 48 mm squares ( 1.9 plus minus 0.03 inches) or 48 x 48 mm(( 1.9 plus minus 0.03 inches)diameter circles Or Sheets can be submitted.

Untreated sample: 48 x 48 mm squares or 48 x 48 diameter circles Or Sheets can be submitted


Turn Around Time: Eight to ten working days are required if commonly used organisms like the one described in AATCC 100 test standards are tested.


To purchase a copy of AATCC 100 test method visit AATCC at AATCC.org

For more information about AATCC 100 antimicrobial textile test, please contact Accugen Laboratories at info@accugenlabs.com

Assessment Of Antimicrobial Finishes On Textile Materials





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Last Updated On: 12-19-2017
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