ISO 22196 Measurement of antibacterial activity on plastics surfaces
ISO 22196 is an internationally recognized test method for evaluating the antibacterial activity of treated plastic materials.
The ISO 22196 test method is used in evaluating the antibacterial activity of antibacterial-treated plastic products and other non-porous materials including paint films and other products.
ISO 22196 is not used to evaluate the effects and propagation of bacteria on non-porous surfaces without antibacterial treatments.
The ISO 22196 test method quantitatively evaluate the antibacterial activity of plastics against the test microorganism over a contact period of 24 hours.
Summary of ISO 22196 Test Method
In ISO 22196 test method, a bacterial suspension is applied to the test specimen and control pieces, in triplicate. Microorganisms inoculated test pieces are then covered with a sterile thin membrane to spread the inoculum, to avoid dehydration and promote good contact between antimicrobial surface and the test organism. Samples are then incubated for 24 hours. Microbial Counts are determined before and after incubation. Reduction of microorganisms is then calculated by using initial count and surviving microorganisms.
ISO 22196 test standard specifies the use of 2 bacterial species (Staphylococcus aureus and Escherichia coli) however test using other bacteria such as MRSA, Pseudomonas, Listeria, Salmonella, Bacillus, Acinebacter etc., as well as fungi such as Aspergillus and Candida can also be carried out.
ISO 22196 test standard specifies an incubation period of 24 hours but other time periods can also be accommodated.
Coating to prepare twelve , 50 x 50 mm size pieces or 12 pieces of 50 x 50 mm size
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